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Huining Liu, Xiaoyin Wang, Ying Zou, Wenbao Wu, Yan Lin, Boyu Ji, Tianyun Wang. Synthetic enhancers including TFREs improve transgene expression in CHO cells. Heliyon, 2024, 10(5):e26901.

Xi Zhang, Yaokun Wang, Dandan Yi, Chi Zhang, Binhuan Ning, Yushun Fu, Yanlong Jia, Tianyun Wang, Xiaoyin Wang. Synergistic promotion of transient transgene expression in CHO cells by PDI/XBP-1s co-transfection and mild hypothermia. Bioprocess Biosyst Eng, 2024, 47(4):557-565.

Mingming Han, Haitong Wang, Huijie Zhang, Jiangtao Lu, Jialiang Guo, Lele Qiu, Xi Zhang, Xiaoyin Wang, Tianyun Wang, Yanlong Jia. A novel dual‑epigenetic inhibitor enhances recombinant monoclonal antibody expression in CHO cells. Appl Microbiol Biotechnol, 2024, 108(1):467.

Zhaoming Cui,Yingying Feng,Yanping Gao,Haitong Wang,Jiangtao Lu,Jialiang Guo,Hongyan Xu,Lele Qiu,Tianyun Wang,Yanlong Jia. Overexpression of YTHDF3 increases the specific productivity of the recombinant protein in CHO cells by promoting the translation process. Biotechnol J. 2024, 19(4): e2400078.

Fu Y, Han Z, Cheng W, Niu S, Wang T, Wang X. Improvement strategies for transient gene expression in mammalian cells. Appl Microbiol Biotechnol. 2024, 108(1): 480.

Shaolei Geng, Ying Zou, Zhiyuan Bai, Min Zhang, Chong Wang, Tianyun Wang. Serum-free medium for recombinant protein expression in insect cells. Biotechnol Appl Biochem, 2024, Online ahead of print.

Zhang Junhe, Yang Wenwen, Zhang Liao, Li Wenqing, Zhang Xi, Wang Xiaoyin, Wang Tianyun. Novel and effective screening system for recombinant protein production in CHO cells. Sci Rep, 2024,14(1): 20856.

Zhang L, Gao JH, Zhang X, Wang XY, Wang TY, Zhang JH. Current strategies for the development of high-yield HEK293 cell lines. Biochem Eng J, 2024, 205: 109279.

张俊河，肖云喜，张辽，潘越，林艳，王天云. 凋亡蛋白酶激活因子1基因敲除HEK293细胞系的构建及其对重组蛋白表达的影响. 中国生物制品学杂志，2024，37 (12)：1421-1427．

张俊河,杨雯雯,王天云.重组蛋白药物真核表达系统的研究进展.中国生物制品学杂志,2024,37(6):756-762.

ShaoLei Geng, Xiaojie Zhao, Xi Zhang, Jihong Zhang, Chunliu Mi, Tianyun Wang. Recombinant therapeutic proteins degradation and overcoming strategies in CHO cells.Applied Microbiology and Biotechnology, 2024, 108:182.

Jihong Zhang, Tianyun Wang*, Chunbo Zhang, Chunliu Mi, Shaolei Geng, Yuanyuan Tang, Xiaoyin Wang. CMV/AAT promoter of MAR-based episomal vector enhanced transgene expression in human hepatic cells. 3 Biotech, 2023, 13: 354.

Xiaoyin Wang, Weili Zhang, Xi Zhang, Yushun Fu, Haomin Wang, Qiuli Sun, Qin Li, Yanlong Jia, Junhe Zhang, Tianyun Wang*. Combination of MAR and intron increase transgene expression of episomal vectors in CHO cells. Biotechnol J, 2023, e2200643.

Fan Liang, Hongyan Xu, Hongwei Cheng, Yabo Zhao, Junhe Zhang*. Patient-derived tumor models: a suitable tool for preclinical studies on esophageal cancer. Cancer Gene Ther, 2023, 10.1038/s41417-023-00652-9.

Yaru Zhao, He Li, Zhenlin Fan, Tianyun Wang*. Effect of Host Cell Protein on Chinese Hamster Ovary Recombinant Protein Production and its Removal Strategies: A Mini Review. Curr Pharm Biotechnol, 2023, 10.2174/1389201024666230818112633.

Qiuli Zhang, Chunliu Mi, Tianyun Wang*. Effects and mechanism of small molecule additives on recombinant protein in CHO cells. Appl Microbiol Biotechnol, 2023, 107(9): 2771-2781.

Yong-Xiao Yang, Qin Li, Wei-Dong Li, Tian-Yun Wang*, Hui-Gen Feng. Factors and Mechanisms Affecting the Secretion of Recombinant Protein in CHO Cells. Curr Pharm Biotechnol, 2023, 24(3):391-400.

Wen-Jing Xu, Yan Lin, Chun-Liu Mi, Jing-Ying Pang, Tian-Yun Wang*. Progress in fed-batch culture for recombinant protein production in CHO cells. Appl Microbiol Biotechnol, 2023, 107(4):1063-1075.

Jiang-Tao Lu, Meng-Ke Xiao, Ying-Ying Feng, Xiao-Yin Wang, Le-Le Qiu, Yu-Rong Chai, Tian-Yun Wang, Yan-Long Jia*. Apilimod enhances specific productivity in recombinant CHO cells through cell cycle arrest and mediation of autophagy. Biotechnol J, 2023, 18(2): e2200147.

Honglin Chen, Rui Zhang, Feng Liu, Changwei Shao, Fangfang Liu, Weidong Li, Jindong Ren, Baolong Niu, Haipeng Liu, Bao Lou. The chromosome-level genome of Cherax quadricarinatus. Sci Data, 2023, 10(1): 215.

Zheng-Mei Li, Zhen-Lin Fan, Xiao-Yin Wang, Tian-Yun Wang. Factors affecting the expression of recombinant protein and improvement strategies in Chinese hamster ovary cells. Front Bioeng Biotechnol, 2022,10:880155.

Yong-Xiao Yang, Zheng-Mei Li, Qin Li, Kai Ma, Yan Lin, Hui-Gen Feng, Tian-Yun Wang. Increase recombinant antibody yields through optimizing vector design and production process in CHO cells. Appl Microbiol Biotechnol, 2022, 106(13-16):4963-4975.

Wen-Wen Yang, Jun-He Zhang, Yun-Yi Xiao, Wen-Qing Li, Tian-Yun Wang. Screening strategies for high-yield Chinese hamster ovary cell clones. Front Bioeng Biotechnol, 2022, 10:858478.

Ting-Ting Xu, Ji-Hong Zhang, Tian-Yun Wang. Progress of Transposon vector system for production of recombinant therapeutic proteins in mammalian cells. Front Bioeng Biotechnol, 106(11):3913-3922.

Mian Wei, Chun-Liu Mi, Chang-Qin Jing, Tian-Yun Wang. Progress of transposon vector system for production of recombinant therapeutic proteins in mammalian cells. Front Bioeng Biotechnol, 2022, 10:879222.

Hui-Ning Liu, Wei-Hua Dong, Yan Lin, Zhao-Hui Zhang, Tian-Yun Wang. The effect of microRNA on the production of recombinant protein in CHO cells and its mechanism.  Front Bioeng Biotechnol, 2022, 10:832065.

Qin Li, Rui-Fang Yan, Yong-Xiao Yang, Chun-Liu Mi, Yan-Long Jia, Tian-Yun Wang. Stabilizing and anti-repressor elements effectively increases transgene expression in transfected CHO cells.  Front Bioeng Biotechnol, 2022, 10:840600.

Yu-Fei Ma, Wei-Dong Li, Kai Ma, Tian-Yun Wang, Hui-Gen Feng. Cell surface markers and their targeted drugs in breast cancer. Curr Protein Pept Sci, 2022, 23(5):335-346.

Chun-Liu Mi, Xiang-Xiang Cao, Kai Ma, Mian Wei, Wei-Jing Xu, Yan Lin, Ji-Hong Zhang, Tian-Yun Wang. Digitoxin promotes apoptosis and inhibits proliferation and migration by reducing HIF-1a and STAT3 in KRAS mutant human colon cancer cells. Chem Biol Interact, 2022, 351:109729.

Wei-Hua Dong, Fang Wang, Qing-Yu Liu, Tian-Yun Wang, Yun Yang, Pei-Xia Guo, Xiang Li, Bing-Di Wei. Downregulation of miRNA-14669 reverses vincristine resistance in colorectal cancer cells through PI3K/AKT signaling pathway. Recent Pat Anticancer Drug Discov, 2022, 17(2):178-186.

Xiao-Yin Wang, Qiu-Jie Du, Wei-Li Zhang, Dan-Hua Xu, Xi Zhang, Yan-Long Jia, Tian-Yun Wang. enhanced transgene expression by optimization of poly A in transfected CHO cells. Front Bioeng Biotechnol, 2022, 10:722722.

Xin-Zhi Miao, Fang Wang, Tian-Yun Wang. Siti Razila Abdul Razak, Muhammad Amir Yunus Ida Shazrina Ismail. Research updates on the clinical implication of long noncoding RNA in digestive system cancers and chemoresintance. 3 Biotech, 2021, 11(9):423. 

Kai Ma, Chun-Liu Mi, Xiang-Xiang Cao, Tian-Yun Wang. Progress of cationic gene delivery reagents for non-viral vector. Appl Microbiol Biotechnol. 2021;105(2):525-538.

Wei-Feng Li, Zhen-Lin Fan, Yan Lin, Tian-Yun Wang. Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells. Front Bioeng Biotechnol. 2021;9:646363.

Huan-Yu Zhang, Zhen-Lin Fan, Tian-Yun Wang. advances of glycometabolism engineering in Chinese hamster ovary cells. Front Bioeng Biotechnol, 2021, 9:774175.

Yuan-Yuan Dou, Yan Lin, Tian-Yun Wang, Xiao-Yin Wang, Yan-Long Jia, Chun-Peng Zhao. The CAG promoter maintains high-level transgene expression in HEK293 cells. FEBS Open Bio Actions Search in PubMed Search in NLM Catalog Add to Search. 2021;11(1):95-104.

李伟风, 樊振林, 张洹瑜, 林艳, 王天云. 用于重组蛋白药物生产的CHO细胞无血清培养基的研究进展. 中国细胞生物学学报. 2021;43(4):905-916.

Xiao Guo, Chong Wang, Tian-Yun Wang. Chromatin-modifying elements for recombinant protein production in mammalian cell systems. Crit Rev Biotechnol. 2020;40(7):1035-1043.

Yan-Mei Li, Meng Wang, Tian-Yun Wang, Yong-Ge Wei, Xiao Guo, Chun-Liu Mi, Chun-Peng Zhao, Xiang-Xiang Cao, Yuan-Yuan Dou. Effects of different 2A peptides on transgene expression mediated by tricistronic vectors in transfected CHO cells. Mol Biol Rep Actions Search in PubMed Search in NLM Catalog Add to Search. 2020;47(1):469-475.

Tian-Yun Wang, Xiao Guo. Expression vector cassette engineering for recombinant therapeutic production in mammalian cell systems. Appl Microbiol Biotechnol. 2020;104(13):5673-5688.

Dan-Dan Yi, Xiao-Yin Wang, Wei-Li Zhang, Meng Wang, Jun-He Zhang, Tian-Yun Wang. Construction of an expression vector mediated by the dual promoter for prokaryotic and mammalian cell expression system. Mol Biol Rep. 2020;47(7): 5185-5190.

Chang-Qin Jing, Meng-Long Guo, Chong Wang, Tian-Jun Ni, Xiao Guo, Tian-Yun Wang. Fusion with matrix attachment regions enhances expression of recombinant protein in human HT-1080 cells. J Biosci Bioeng. 2020;130(5):533-538.

Jun-He Zhang, Ji-Hong Zhang, Xiao-Yin Wang, Dan-Hua Xu, Tian-Yun Wang. Distance effect characteristic of the matrix attachment region increases recombinant protein expression in Chinese hamster ovary cells. Biotechnol Lett. 2020 Feb;42(2):187-196.

Xiao-Yin Wang, Wei-Li Zhang, Yan-Long Jia, Meng Wang, Dan-Dan Yi, Tian-Yun Wang. Woodchuck hepatitis post-transcriptional regulatory element improves transgene expression and stability mediated by episomal vectors in CHO-K1 cells. Acta Biochim Biophys Sin (Shanghai). 2020;52(11):1285-1288.

Ji-Hong Zhang, Jun-He Zhang, Shan Cheng, Wen-Wen Yang, Shi-Jiang Li. Enhanced transgene expression using two β-globin MARs flanking expression cassettes in stably transfected CHO-K1 cells. 3 Biotech. 2019;9(11):435.

Xiao-Yin Wang, Xi Zhang, Tian-Yun Wang, Yan-Long Jia, Dan-Hua Xu, Dan-Dan Yi. Shortened nuclear matrix attachment regions are sufficient for replication and maintenance of episomes in mammalian cells. Mol Biol Cell. 2019;30(22):2761-2770.

Yan-Long Jia, Xiao Guo, Tian-Jun Ni, Jiang-Tao Lu, Xiao-Yin Wang, Tian-Yun Wang. Novel short synthetic matrix attachment region for enhancing transgenic expression in recombinant Chinese hamster ovary cells. J Cell Biochem. 2019;120(10):18478-18486.

Xiao-Yin Wang, Dan-Dan Yi, Tian-Yun Wang, Yan-Fang Wu, Yu-Rong Chai, Dan-Hua Xu, Chun-Peng Zhao, Chao Song. Enhancing expression level and stability of transgene mediated by episomal vector via buffering DNA methyltransferase in transfected CHO cells. J Cell Biochem. 2019;120(9):15661-15670.

Yan-Long Jia, Xiao Guo, Xi-Cheng Wang, Tian-Yun Wang. Human genome-derived TOP1 matrix attachment region enhances transgene expression in the transfected CHO cells. Biotechnol Lett. 2019;41(6-7):701-709.

王稳, 王天云. CHO细胞表达系统启动子. 中国生物化学与分子生物学报. 2019;35(11):1175-1182.

窦媛媛, 林艳, 高向征, 王燕芳, 王小引, 王天云. 重组人bFGF的原核表达及功能分析. 中国细胞生物学学报. 2019;41(7):1365-1370.

董卫华, 李翠萍, 杨赟, 王天云, 王芳. 不同方向内含子对重组CHO细胞中神经因子表达的影响. 生物工程学报. 2019;35(6):1071-1078.